ABSTRACT
Objective To improve the quality standard of Flos Chrysanthemum Indicum from diffrent origins by analyzing on linarin and cumambrin A qualitatively and quantitatively. Methods Qualitative analysis of linarin and cumambrin A was carried out by thin layer chromatography (TLC); Content determination of linarin and cumambrin A was using high performance liquid chromatography (HPLC) on YMC C18 column (250 mm × 4.6 mm, 5 μm); The mobile phase was a mixture of acetonitrile-0.05% phosphoric acid solution; The elution mode was gradient system (0-18 min, 25%-26% A; 18-26 min, 26%-32% A; 26-33 min, 32%-34% A; 33-35 min, 34%-40% A; 35-65 min, 40%-50% A); The flow rate was 0.8 mL/min; The detection wavelengths were 203 nm and 340 nm; The column temperature was 35 ℃. Results Linarin and cumambrin A by TLC was obvious. The concents of linarin and cumambrin A in Flos Chrysanthemum Indicum from diffrent origins were different. The concents of linarin and cumambrin A from Xinyang were the highest (6.53% and 0.81% respectively). Conclusion It is the first time to establish a method to evaluate different components in Flos Chrysanthemum Indicum by TLC and HPLC. The method is simple, accurate and reproducible, which can effectively improve the existing quality standard of Flos Chrysanthemum Indicum. The result also showed Linarin and cumambrin A could reflect the quality of Flos Chrysanthemum Indicum.